For purification of up to 10 mg transfectiongrade plasmid or cosmid dna. Plasmid dna was eluted in 2 ml volume and quantified using thermo scientifics nanodrop 2000c system. More than 400 g of plasmid dna is quantified with a spectrophotometer. The endofree plasmid miniprep kit provides an efficient endotoxin removal step into the traditional purification procedure to produce transfection grade plasmid dna. Does anyone have help with problems in purifying a. The pureyield plasmid maxiprep system is designed to isolate highquality. Sample preparation aurum plasmid mini kit supports any downstream research with 99% plasmid dna purity requires plasmid preparation kits. Thermo scientific genejet endofree plasmid maxiprep kit is a fast, convenient, and efficient system for the large scale purification of endotoxinfree plasmid dna. Mix gently by inverting the tube 46 times and incubate at room temperature for exactly 5 min. Omega biotek inc endofree plasmid maxi kit manufacturer. This kit is designed for fast and efficient purification of plasmid dna from 3 to 12 ml of e.
Endotoxins are major contaminants found in plasmid dna preparations. Plasmid maxi kit is the maxi scale system to deliver highquality plasmid dna using the spin column format, without costly accessories. These are available online in convenient and compact pdf format at. Endofree maxi plasmid kit is based on alkaline lysis technology followed by adsorption of dna onto silica membrane in the presence of high salt, and uses buffer p4 and filtration cs1 to wipe off the endotoxin and protein impurity effectively. Highspeed plasmid mini kit fast and economical plasmid minipreps the highspeed plasmid mini kit is designed for the rapid isolation of plasmid or cosmid dna from 14 ml of bacterial cultures. The geneaid midi plasmid kit uses prepacked anionexchange resin columns to purify plasmid dna from 50300 ml of cultured bacterial cells. Endofree plasmid mini kit i endofree plasmid mini kit ii endofree plasmid midi kit endofree plasmid maxi kit 15 ml 515 ml 2550 ml 30 g 70 g 250 g. Highspeed plasmid mini kit 300 300 preps pd300 highspeed plasmid mini kit 100 100 preps pd100 product name package size cat. Solution i once rnase a is added should be stored at 28. Endofree plasmid maxiprep kit syringe format notice. Qiagen plasmid maxi kit 25, 12163 from qiagen labsave. The tonkbio endofree plasmid maxi kit is designed for efficient and convenient extraction of endofree dna. Hispeed plasmid purification handbook harvard university. The kit is able to purify plasmids up to,000 bp in size, and typical yields from a 100 ml culture for a high copy number plasmid are between 0.
The kits for pcr cleanup, gel purification, and column based plasmid kits are pretty similar in the final portion of the protocols, but differ in the sample handling early on, primarily in releasing the dna and adjusting chemistry so that it binds. Plasmid maxi prep kit catalogue number of the product. What is your preferred maxi prep kit for plasmid dna grown in bacteria. Endofree plasmid maxi kit 10, 12362 from qiagen labsave. Endofree plasmid maxi kit 10 from qiagen selectscience. Timerun, 500g yield, high yields of highcopy plasmid dna, for purification of up to. Qiafilterplasmidpurificationhandbook 042012 7 introduction qiafilter plasmid kits are based on the remarkable selectivity of patented qiagen resin. Hispeed plasmid midi and maxi kits protocol before staring make sure that rnase a has been added to p1 buffer. All other materials should be stored at room temperature. Endofree plasmid maxi kit en print bookmark share pdf 50kb english format file size language. Plasmid dna maxiprep kits thermo fisher scientific ru.
When transferred into filter syringelet stand the lysate at room, temperature for 1 to 2 min until the white precipitates float to the top, then press the plunger. The purelink hipure plasmid filter maxiprep kit is designed to isolate plasmid dna of the highest purity from 100 to 500 ml of an overnight bacterial culture. Does anyone have help with problems in purifying a lentivirus backbone plasmid with maxi prep. The results illustrate the very high binding capacity of the columns in the isolate ii plasmid mini kit. The isolation protocol and buffer formulations were optimized for fast plasmid culture screening omitting the time consuming cell pelleting procedure. Although yields vary according to plasmid copy number, li strain, and conditions of. The genopure plasmid maxi kit is used to prepare purified plasmid dna in larges quantities. Qiagen endofree plasmid maxi kit, 100l to 250ml culture volume, anionexchange technology, transfectiongrade plasmid or cosmid dna purification, manual processing, 150 min. The hibind dna maxi column facilitates the binding, washing, and elution steps, thus enabling multiple samples to be simultaneously processed. They are not to be used for human diagnostic or drug purposes or to be administered to humans unless expressly cleared for that purpose by the food and drug. Description the accuprep nanoplus plasmid mini, midi and maxi extraction kit was developed for the extraction of highly purified plasmid dna from cultured bacterial cells within 10 min.
Mar 14, 2007 finally, these kits are slightly more expensive than some of the other kits available on the market. With qiagens plasmid maxi kit, we get reliable results with good yields of pure plasmid dna. Maxi the overall principle combines modified alkaline. For transfection of sensitive cell lines endotoxins are a major pr. To help you find what youre looking for, weve gathered our plasmid kits on one page. Accuprep nanoplus plasmid extraction kit technical manual i.
Toxout endofree plasmid mini kit k26 biovision, inc. The kit allows for the isolation of plasmid dna with final endotoxin levels of 0. Solution ii must be tightly capped when not in use. For purification of up to 10 mg endotoxinfree advanced transfectiongrade plasmid or cosmid dna. Trueblue lysis buffer an optional color indicator is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. Centrifugation tubes or vessels with suitable capacity for the volumes specified in the appropriate protocol. Endofree maxi plasmid kit handbook 2 introduction endofree maxi plasmid kit is based on alkaline lysis technology followed by adsorption of dna onto silica membrane in the presence of high salt, and uses buffer p4 and filtration column cs to wipe off the endotoxin and protein impurity effectively. Plasmid dna is then further purified by adsorbing to the hibinda silica membrane. Which is the best inhouse protocol for midi and maxi prep plasmid.
What is your preferred maxi prep kit for plasmid dna grown in. Omega biotek inc endofree plasmid maxi kit endofree. Plasmid maxiprep kit catalogue number of the product. Quick and cost effective, most widely accepted protocol for transfection, rapid. This system applies salt free reagent to get high pure plasimd, no salt remain, and the concentration of endotoxin below 0. Jul 17, 2007 the qiagen plasmid maxi kit is designed to perform purification of plasmids up to approximately 150 kb, with possible reduced elution efficiencies for constructs larger than 4550 kb.
Plasmid dna mini kit i and plasmid dna mini kit ii components are guaranteed for at least 12 months from the date of purchase when stored as follows. Plasmid dna prepared by endofree plasmid kit is suitable for a variety of routine. The endofree plasmid maxi kits provide an efficient method for the largescale isolation of endotoxinfree plasmid dna. Qiagen plasmid purification handbook qiagen plasmid midi, maxi, mega, and giga kits qiafilter plasmid midi, maxi, mega, and giga kits endofree plasmid maxi, mega, and giga kits for purification of ultrapure plasmid dna second edition august 2003. Highthroughput and efficient purification of high quality plasmid dna from recombinant escherichia coli strains in 96well plates format. When properly stored, all kit components are stable until the expiration date printed on the label.
The table below will help you choose the right plasmid dna maxiprep kit. The quality of the fast ion plasmid maxi kit is tested on a lottolot basis by isolating plasmid dna from a 100 ml overnight e. The purelink hipure plasmid filter kits include hipure filter columns, which combine filtration for clarification of the bacterial lysate wit. Qiagen plasmid maxi kit, 25 preps, 100 to 500ml culture volume, transfectiongrade plasmid or cosmid dna purification, anionexchange technology, 500g yield, 160 min. Maxi plasmid dna kit pm010, pm025 the geneaid maxiprep plasmid kit was designed for plasmid dna purification using prepacked anionexchange resin maxiprep plasmid columns to purify plasmid dna from 2001600 ml of cultured bacterial cells. Endofree plasmid midi kit and the endofree plasmid maxi kit combine the power of hibind technology with omega bioteks innovative endotoxin removal technology etr to deliver highquality plasmid dna with low endotoxin levels for use in eukaryotic transfection, and in vitro experiments. Biovisions toxouttm endofree ezfilter plasmid maxi kit is designed for fast and efficient purification of plasmid dna from 150400 ml of e. Our technical services departments are staffed by experienced scientists with extensive. Qiagen plasmid maxi kit has been developed to purify either high or lowcopy plasmids and cosmids. The isolation procedure is based on a modified alkaline lysis protocol and can be divided into. Pdf zusammenfugen pdfdateien online kostenlos zu kombinieren. Omega biotek inc d692603 this product was recently added by customer request, and is available for your convenience. Following lysis, endotoxins are removed from the cleared lysate with the addition of etr solution.
A protocol for isolation of plasmid dna for use in eukaryotic transfection and. Endofree plasmid maxi kit following manufacturers recommended protocol. Plasmid kits addgenes repository contains over 80,000 plasmids, which includes over 90 kits. I have not done a direct price comparison as i havent found a good reason to switch to a different kit. Add 10 ml of buffer p3, mix gently, incubate on ice for 1520 min. The purified plasmid 1 g is used in ecor i digestion, and checked by. Easypreptm endofree plasmid table of contents miniprep ii manual. Identification of the preparationsubstance and of the company preparation name. Qiagen plasmid purification handbook harvard university. The maxi column has a dna binding capacity of 1200 g. Invitrogen plasmid maxiprep kits offer a range options to eliminate the challenges. Qiagen and purelink sell mini prep versions of the dripgravity columns that work identical to the midimaxi prep.